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  • PRODUCTS - HAISHENGJIE CRYOGENIC EQUIPMENT

    Overview: The transport storage series are economical small liquid nitrogen tanks designed specifically for biological samples static storage and for transporting biological samples.The series is made of high strength lightweight aluminum alloy, containing multi-layer insulation to make the product safe, lightweight and efficient, and it has a variety of accessories optional.

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    Taylor-Wharton® produces a comprehensive range of cryogenic equipment for storage, transportation and regasification purpose cater to all aspects of the cold chain supply for Industrial Gas, Life Sciences and LNG applications. Its products are used in trucking, marine, mine haul, liquefaction, oil and gas, drilling, municipalities, industrial ...

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    Cell samples should be stored in vapor phase liquid nitrogen below –135°C. Always use sterile cryovials for storing frozen cells. Cryovials containing the frozen cells may be stored immersed in liquid nitrogen or in the gas phase above the liquid nitrogen (see Safety Note below). Always wear personal protective equipment.

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    The storage of stem cells is therefore a key component in facilitating the commercialization of cellular therapies. Cryopreservation (ie, freezing) of both the starting materials and final product is typically required in order to preserve the cellular function that is often lost in long-term culture [4].

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    This cell culture procedure enables the production of a working cell bank (WCB) of 100 vials of 1 × 10 6 iPSCs each within 10-12 days from a cryopreserved vial of iPSC of a master cell bank (MCB). It is optimized for manufacturing large numbers of cells at reduced cost by allowing industrial scale production of iPSCs in a time-efficient manner.

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    The initial cooling rate was −2 °C/min to −9 °C with manual seeding performed and cooling was continued at different ramps to−140 °C, when the samples were plunged into liquid nitrogen at −196 °C, and transferred to a liquid nitrogen storage tank.

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    1. Detection of post copilatory plus- post breeding a plus will be detectable by observing the vagina for a thick shiny plug which is a mixture of mucous, semen and vaginal secretions. 2. vaginal smear - by injecting a small volume of sterile saline into the vagina and drawing it back out one can microscopically evaluate for presence of semen.

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